منابع مشابه
Transformation and microinjection*
Copyright: © 2006 Thomas C. Evans and contributors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and contributors reproduction in any medium, provided the original author and source are credited. To whom correspondence should be addressed. E-mail: [email protected] Transformation and micro...
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متن کاملMicroinjection of Specific DNA Sequences
The mutant Paramecium tetraurelia cell line d48 is unable to express the serotype A protein on its surface. Although the A gene is intact in the micronuclei of d48, the A gene copies in the macronucleus contain a large deletion eliminating virtually the entire coding sequence. Previous studies showed that microinjection of a plasmid containing the entire A gene into the macronucleus of d48 perm...
متن کاملMicrofluidic based single cell microinjection.
We report a microfluidic based approach for single cell microinjection in which fluid streams direct a cell onto a fixed microneedle in contrast to moving a microneedle towards an immobilized cell, as done in conventional methods. The approach simplifies microinjection and offers the potential for flow through automated microinjection of cells.
متن کاملChromosome-mediated Transformation via Microinjection
Many economically important plant characteristics such as drought tolerance are encoded by multiple genes or polygenes. Individually, polygenes exert very little effect on the phenotype [14]. These genes can either occur scattered throughout the genome or clustered within blocks. The genetic methods for analyzing polygenic traits have been thoroughly developed [17]. The prohibitively large numb...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2018
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot096974